Recombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli

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dc.contributor.authorBui, Cong Chinh
dc.contributor.authorHo, Ta Giap
dc.contributor.authorVu, Van Van
dc.date.accessioned2024-08-24T01:56:02Z
dc.date.accessioned2024-08-29T02:16:44Z
dc.date.available2024-08-24T01:56:02Z
dc.date.available2024-08-29T02:16:44Z
dc.date.issued2022
dc.description5 p.
dc.description.abstractMGG06069 is a polysaccharide monooxygenase protein that originated from the fungus that causes rice blast, Magnaporthe oryzae. It has the potential to speed up the cellulolysis process, which can be beneficial for industry. In this study, the full-length MGG06069 protein and its polysaccharide monooxygenase domain were expressed in Escherichia coli in order to produce recombinant proteins on a large scale for further enzymatic experiment. It was shown that the amount of the recombinant polysaccharide monooxygenase domain protein was much higher compared to that of the full-length protein. In addition, only one band of expected size was obtained for the recombinant PMO domain protein. These results suggest the purified polysaccharide monooxygenase domain of MGG06069 that can be used for enzymatic assays in the future.
dc.identifier.citationNguyen Tat Thanh University. (2022). Journal of Science and Technology - NTTU, (17). ISSN 2615-9015.
dc.identifier.issn2615-9015
dc.identifier.urihttps://repository.ntt.edu.vn/handle/298300331/50160
dc.language.isovi_VN
dc.publisherNguyen Tat Thanh University
dc.relation.ispartofseriesJournal of Science and Technology - NTTU; No. 17
dc.subjectAA9
dc.subjectMGG06069
dc.subjectEscherichia coli
dc.subjectPolysaccharide Monooxygenase
dc.subjectPMOs
dc.subjectHoạt tính enzym
dc.titleRecombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli
dc.typeArticle

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