Development of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease-causing PirAB toxins in shrimp

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dc.contributor.authorTran, Van Hieu
dc.contributor.authorDuong, Ngoc Diem
dc.contributor.authorNguyen, Phuoc Khai Hoan
dc.contributor.authorDo, Thi Kim Yen
dc.contributor.authorHuynh, Tuan Binh
dc.contributor.authorMai, Hoang Thuy Dung
dc.contributor.authorNguyen, Thi Nguyet Thu
dc.contributor.authorTran, Linh Thuoc
dc.date.accessioned2024-08-22T08:05:31Z
dc.date.accessioned2024-08-29T02:19:25Z
dc.date.available2024-08-22T08:05:31Z
dc.date.available2024-08-29T02:19:25Z
dc.date.issued2023
dc.description7 tr.
dc.description.abstractAcute hepatopancreatic necrosis disease (AHPND) is currently the most affected disease of shrimp farms worlwide As rapid and specific detection methods are essential to improve disease management, a lateral flow immunoassay (LFIA) was designed for the detection of PirAvp and PirBvp toxins in shrimp. In this study, polyclonal antibody based lateral flow immunoassay (LFIA) strip has been developed. The recombinant PirAvp and PirBvp toxin‐like proteins of Vibrio parahaemolyticus (VPAHPND) was used to immunize rabbits with the highest antibody titre after the third booster. This polyclonal rabbit antiserum was purified and used to develop a LFIA strip for the detection of AHPND pathogen. This test strip could detect PirAvp and PirBvp toxins up to 125 ng/mL by naked eye within 15 min. No cross-reactivity was observed with VPnon-AHPND. Furthermore, the sensitivity and specificity of LFIA were 94.0% and 98.0%, respectively. This LFIA strip could be used as a handy tool by the farmers and technicians in situ.
dc.identifier.citationNguyen Tat Thanh University. (2023). Journal of Science and Technology - NTTU, Volume 6, Issue 4. ISSN 2615-9015.
dc.identifier.issn2615-9015
dc.identifier.urihttps://repository.ntt.edu.vn/handle/298300331/50349
dc.language.isoen
dc.publisherNguyen Tat Thanh University
dc.relation.ispartofseriesJournal of Science and Technology - NTTU; Vol. 6, No. 4
dc.subjectAHPND
dc.subjectPolyclonal antibody
dc.subjectPirAvp and PirBvp toxins
dc.subjectLateral flow immunoassay strip
dc.titleDevelopment of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease-causing PirAB toxins in shrimp
dc.typeArticle
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