Duyệt theo Tác giả "Phung, Thi Thu Huong"

Đang hiển thị 1 - 2 của tổng số 2 kết quả
  • Hình ảnh thu nhỏ
    Tài liệu
    Detecting Fasciola hepatica and F. gigantica microRNAs using loop-mediated isothermal amplification (LAMP)
    (Nguyen Tat Thanh University, 2019) Tran, Hong Diem; Phung, Thi Thu Huong
    Trematodo, genus Fasciola, named Fasciola hepatica and F. gigantica. The widespread appearance of these species in water and food makes fascioliasis become a global zoonotic disease that affects 2.4 million people in more than 75 countries worldwide. Typically, F. hepatica and F. gigantica can be recognized with parasitological techniques to detect Fasciola spp. eggs, immunological techniques to detect worm-specific antibodies, or molecular techniques for instance polymerase chain reactions to detect parasitic genomic DNA. Recently, miRNAs have been recognised a key regulator and potential diagnostic biomarkers of diseases, including parasitic infection. An isothermal PCR called LAMP (loop-mediated isothermal amplification) is rapid, sensitive, and this amplification is very extensive, making it well-suited for field diagnostics. LAMP reaction for miRNA detection has been introduced and is able to detect miRNA in the range between 1.0amol and 1.0pmol, showing high selectivity to differentiate one miRNA sequence from others. Here, we introduced a modified LAMP to detect a typical miRNA of both F. hepatica and F. gigantica. Our method does not demand an initial heating step and the reactions have a high sensitivity even 1,000 times higher in comparison to that reported in previous studies. These results create a promising technique basis for some novel and simple device to diagnose fascioliasis and other parasitic diseases at point-of-care.
  • Hình ảnh thu nhỏ
    Tài liệu
    Purification of Saccharomyces cerevisiae recombinant Crp1
    (Nguyen Tat Thanh University, 2018) Phung, Thi Thu Huong; Tran, Hong Diem
    A complex Mus81-Mm4 is a DNA structure–specific endonuclease in Saccharomyces cerevisiae. Mus81-Mms4 functions in processing of recombination intermediates that could arise during the repair of stalled and blocked replication forks and double stranded breaks. Mus81-Mms4 works with many proteins involved in DNA repair, replication fork stability, and joint molecule formation/resolution during homologous recombination repair. A biochemical screening of protein(s) that enhances the Mus81-Mms4 endonuclease activity on its preferable substrates in vitro revealed that Crp1, a cruciform DNA-recognizing protein, which can specifically bind to DNA four-way junction structures like Holliday junctions could be the potential factor. To further demonstrate that Crp1 interacts functionally with Mus81-Mms4 in vitro, we carried out the purification of recombinant Crp1 using Escherichia coli system. Our results showed that the purified Crp1 was highly homogenous and active that is ready for biochemical use.