Duyệt theo Tác giả "Nguyen, Huu Hung"

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  • Hình ảnh thu nhỏ
    Tài liệu
    A simple non-enzymatic protocol for isolation and culture of mesenchymal stem cells from adipose tissues
    (Nguyen Tat Thanh University, 2018) Nguyen, Thi Phuong; Nguyen, Huu Hung
    Mesenchymal stem cells (MSCs) are multipotent stem cells – ability to differentiate into several cell types. Previous studies have showed a high number of these cells can be found in adipose tissue. This study has established a simple protocol for isolation and culture of MSCs from mouse adipose tissue using minimal requirements. With non-enzymatic approach, we successfully isolated and cultured the MSCs. Isolated MSCs were identified through morphology and ability of differentiation into adipocyte-like cells under the presense of stimuli in the culture medium.
  • Hình ảnh thu nhỏ
    Tài liệu
    An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow
    (Nguyen Tat Thanh University, 2018) Nguyen, Thi Phuong; Nguyen, Huu Hung
    Bone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure.