Duyệt theo Tác giả "Ho, Ta Giap"

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  • Hình ảnh thu nhỏ
    Tài liệu
    Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B
    (Trường Đại học Nguyễn Tất Thành, 2020) Ho, Ta Giap; Ngo, Nguyen Vu; Vu, Van Van
    Polysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production. Here we cloned the NCU08746 gene, an AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae.
  • Hình ảnh thu nhỏ
    Tài liệu
    Recombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli
    (Nguyen Tat Thanh University, 2022) Bui, Cong Chinh; Ho, Ta Giap; Vu, Van Van
    MGG06069 is a polysaccharide monooxygenase protein that originated from the fungus that causes rice blast, Magnaporthe oryzae. It has the potential to speed up the cellulolysis process, which can be beneficial for industry. In this study, the full-length MGG06069 protein and its polysaccharide monooxygenase domain were expressed in Escherichia coli in order to produce recombinant proteins on a large scale for further enzymatic experiment. It was shown that the amount of the recombinant polysaccharide monooxygenase domain protein was much higher compared to that of the full-length protein. In addition, only one band of expected size was obtained for the recombinant PMO domain protein. These results suggest the purified polysaccharide monooxygenase domain of MGG06069 that can be used for enzymatic assays in the future.